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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

28卷

期数:

2022年04期

页码:

390-397

栏目:

基础医学

出版日期:

2022-07-20

文章信息/Info

Title:

Study on central carbon metabolism pathway in head and neck squamous cell carcinoma

文章编号:

1006-8147（2022）04-0390-08

作者:

黄鑫¹; 闫申²; 阮宏莹¹; 3

（1.天津医科大学一中心临床学院，天津 300192；2.天津医科大学口腔医学院，天津 300070；3. 中南大学湘雅医学院附属海口医院耳鼻咽喉头颈外科，海口570000）

Author(s):

HUANG Xin¹; YAN Shen²; RUAN Hong-ying¹; 3

（1.First Central Clinical School，Tianjin Medical University，Tianjin 300192，China；2.College of Stomatology，Tianjin Medical University，Tianjin 300070，China；3. Department of Otolaryngology-Head and Neck Surgery，Haikou Affiliated Hospital of Central South University Xiangya School of Medicine，Haikou 570000，China）

关键词:

头颈鳞状细胞癌; 转录组学; 蛋白质组学; 代谢组学; 平行反应监测

Keywords:

head and neck squamous cell carcinoma;  transcriptomics;  proteomics;  metabolomics;  parallel reaction monitoring

分类号:

R762

DOI:

-

文献标志码:

A

摘要:

目的：探究头颈鳞癌代谢重编程机制。方法：通过对头颈鳞癌和癌旁组织进行真核有参转录组学、TMT标记定量蛋白质组学和非靶向代谢组学分析，找到差异表达基因、蛋白质和代谢物，进行基因本体（GO）富集分析和京都基因和基因组百科全书（KEGG）富集分析等生物信息学分析，两两组学联合分析预测参与头颈鳞癌代谢重编程的重要代谢通路。然后进行平行反应监测（PRM）验证通路重要蛋白，找到差异表达蛋白及代谢通路。结果：联合分析结果提示，肿瘤中心碳代谢和蛋白质消化吸收途径可能与肿瘤有关。PRM结果表明，表皮生长因子受体（EGFR）、乳酸脱氢酶A（LDHA）、磷酸甘油酸酯转位酶1（PGAM1）、己糖激酶3（HK3）和磷酸果糖激酶（PFKP）在肿瘤中表达升高并参与肿瘤中心碳代谢途径。结论：肿瘤中心碳代谢通路及相关蛋白EGFR、 PGAM1、 LDHA、 HK3、 PFKP在头颈鳞癌发生、发展中起关键作用。

Abstract:

Objective： To investigate the metabolic mechanisms of head and neck squamous cell carcinoma（HNSCC）. Methods: Eukaryotic transcriptomics，TMT labeled quantitative proteomics，and untargeted metabolomics on head and neck squamous cell carcinoma and adjacent tissues were performed to identify differentially expressed genes，proteins，and metabolites. Gene ontology（GO） enrichment analysis，Kyoto Encyclopedia of Genes and Genomes（KEGG） enrichment analysis，and other bioinformatics analyses were performed. Integration analyses were carried out to predict important pathways involved in metabolic reprogramming in HNSCC and perform parallel reaction monitoring（PRM） were used to identify differentially expressed proteins and metabolic pathways. Results: Integration analyses indicated central carbon metabolism in cancer and protein digestion and absorption pathway might be relevant to tumorigenesis. In addition，the results of PRM clarified significantly elevated levels of epidermal growth factor receptor（EGFR），phosphoglycerate mutase 1（PGAM1），lactate dehydrogenase A（LDHA），Hexokinase 3（HK3），and phosphofructokinase（PFKP），which were consistent with central carbon metabolism. Conclusion: Central carbon metabolism in cancer is the critical metabolomic pathway in which EGFR，PGAM1，LDHA，HK3，PFKP play essential roles in the development of HNSCC.

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相似文献/References:

备注/Memo

备注/Memo:

作者简介:黄鑫（1996-），硕士在读，研究方向：头颈鳞癌代谢编程；通信作者：阮宏莹，E-mail：hongyingruan@tmu.edu.cn。

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更新日期/Last Update: 2022-07-20