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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

28卷

期数:

2022年04期

页码:

383-389

栏目:

基础医学

出版日期:

2022-07-20

文章信息/Info

Title:

Analyze the expression and clinical significance of MED19 gene in hepatocellular carcinoma based on TCGA database

文章编号:

1006-8147（2022）04-0383-07

作者:

武燕洁; 李譞媛; 宣成昊; 兰蓓

（天津医科大学基础医学院生物化学与分子生物学系，天津300070）

Author(s):

WU Yan-jie; LI Xuan-yuan; XUAN Cheng-hao; LAN Bei

（Department of Biochemistry and Molecular Biology，School of Basic Medical Sciences，Tianjin Medical University，Tianjin 300070，China）

关键词:

TCGA; 肝癌; MED19; 基因表达分析

Keywords:

TCGA database; hepatocellular carcinoma; MED19; gene expression analysis

分类号:

R735.7

DOI:

-

文献标志码:

A

摘要:

目的：探讨MED19在肝癌患者中的表达及与预后的关系。方法：基于TCGA数据库收集相关数据，挖掘MED19在肝癌组织和正常组织、不同临床特征和免疫分型中的表达差异。比较MED19在免疫分型中的表达差异，并基于“ESTIMATE”算法分析其表达水平与肿瘤微环境的相关性。通过Kaplan-Meier法以及Cox回归分析MED19表达量对肝癌患者生存期的影响。通过基因集富集分析（GSEA）并且筛选差异基因进行基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析，探讨MED19的表达对其他基因集的影响。利用Cellminer数据库数据分析MED19表达量与药物敏感性的关系。结果：在肿瘤组织中MED19的表达增加（W=1 422，P＜0.001），且MED19表达量与临床分期（stage Ⅱ vs. stage I：W=5 828，P＜0.01，stage Ⅲ vs. stage I，W=5707，P＜0.01）、病理分级（G3 vs. G1：W=2 656，P＜0.05）和T分期（T2 vs. T1：W=6 485，P＜0.01，T3 vs. T1：W=5 758，P＜0.01，T4 vs. T1：W=770，P＜0.05）相关。不同免疫亚型中MED19的表达存在差异，且MED19高表达引起基质得分减少（W=14 363，P＜0.01）。MED19高表达使肝癌患者生存率下降（χ2=11.7，P＜0.01），且是患者生存的独立危险因素（HR=1.123，P＜0.01）。GSEA结果显示MDE19高表达组在癌症相关通路富集，此外MED19高表达组和低表达组差异基因的GO和KEGG分析表明，MED19高表达组中的上调表达基因也主要在细胞周期等相关通路富集。药物敏感性分析显示MED19在癌症中高表达与克拉屈滨（r=0.331，t=2.671，P=0.010）、白屈菜红碱（r=0.313，t=2.507，P=0.015）等药物的敏感性呈正相关。结论：MED19在肝癌中表达升高，且与肝癌的预后不良相关。

Abstract:

Objective： To investigate the expression and prognosis of MED19 in patients with hepatocellular carcinoma （HCC）. Methods: Relevant data were collected based on TCGA database to explore the expression differences of MED19 in immune types between HCC tissues and normal tissues，as well as in different clinical characteristics. The expression differences of MED19 in immune types were compared，and the correlation between the expression level of MED19 and tumor microenvironment was analyzed based on "ESTIMATE" algorithm. Kaplan-Meier method and Cox regression were used to analyze the influence of MED19 expression level on the survival of HCC patients. Gene set enrichment analysis（GSEA），Gene Ontology（GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） enrichment analysis were used to investigate the effect of MED19 expression on other gene sets. The relationship between MED19 expression level and drug sensitivity was analyzed by Cellminer database data. Results: The expression of MED19 increased in tumor tissues（W=1 422，P<0.001），and the expression level of MED19 was correlated with clinical stage（Stage Ⅱ vs. Stage I: W=5 828，stage Ⅲ vs. Stage Ⅰ，W=5 707， P<0.01），pathological grading（G3 vs. G1: W=2 656，P<0.05） and T staging （T2 vs. T1: W=6 485，P<0.01，T3 vs. T1: W=5 758，P<0.01，T4 vs. T1：W=770，P<0.05）. The expression of MED19 was different in different immune subtypes，and the high expression of MED19 caused the decrease of matrix score（W=14 363，P<0.01）. The high expression of MED19 decreased the survival rate of HCC patients （χ2=11.7，P<0.01） and was an independent risk factor for survival of patients（HR=1.123，P<0.01）. GSEA results showed that the high expression group of MDE19 was enriched in cancer-related pathways. In addition，GO and KEGG analysis of the differential genes screened out from the high expression group and the low expression group of MED19 showed that the up-regulation genes in the high expression group of MED19 were mainly enriched in cell cycle-related pathways. Drug sensitivity analysis showed that the high expression of MED19 in cancer was positively correlated with the sensitivity of clattrobin（r=0.331，t=2.671，P=0.010），chelidonine（r=0.313，t=2.507，P=0.015） and other drugs. Conclusion: The expression of MED19 is elevated in HCC and is associated with poor prognosis of hepatocellular carcinoma.

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备注/Memo

备注/Memo:

作者简介:武燕洁（1996-），女，硕士在读，研究方向：肿瘤表观遗传学；通信作者：宣成昊，E-mail：chenghaoxuan@tmu.edu.cn；兰蓓，E-mail：lanpei@tmu.edu.cn。

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更新日期/Last Update: 2022-07-20