|本期目录/Table of Contents|

[1]王 娅,庄布军,吴伯岳,等.Mn-ZnS量子点室温磷光检测尿液中硫酸庆大霉素[J].天津医科大学学报,2018,24(01):68-72.
 WANG Ya,ZHUANG Bu-jun,WU Bo-yue,et al.Determination of gentamycin sulfat in urine by room-temperature phosphorescence sensor based on Mn-ZnS quantum dots[J].Journal of Tianjin Medical University,2018,24(01):68-72.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
24卷
期数:
2018年01期
页码:
68-72
栏目:
技术与方法
出版日期:
2018-01-20

文章信息/Info

Title:
Determination of gentamycin sulfat in urine by room-temperature phosphorescence sensor based on Mn-ZnS quantum dots
文章编号:
1006-8147(2018)01-0068-05
作者:
(1. 天津医科大学第二医院药学部,天津 300211;2.天津医科大学药理学系,天津 300070;3.天津医科大学药学院,天津 300070)
Author(s):
WANG Ya12ZHUANG Bu-jun2WU Bo-yue3GAO Wei-zhen23

(1.Department of Pharmacy, TheSecond Hospital, Tianjin Medical University,Tianjin 300211,China;2.Department of Pharmacology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China; 3. College of Pharmacy,, Tianjin Medical University, Tianjin 300070, China)

关键词:

量子点磷光法检测硫酸庆大霉素

Keywords:

quantum dots phosphorescencedetectiongentamicin sulfate

分类号:
R927.2
DOI:
-
文献标志码:
A
摘要:

目的:基于硫酸庆大霉素对Mn掺杂ZnS量子点的磷光增敏作用,建立了一种简便且灵敏测定硫酸庆大霉素浓度的新方法。方法:在水相中合成巯基丙酸(MPA)包裹的Mn掺杂ZnS量子点,实验中考察了缓冲溶液pH和类型、反应时间及量子点浓度等因素对室温磷光检测的影响。利用荧光分光光度计通过测定Mn掺杂ZnS量子点磷光强度的变化,实现对尿液中硫酸庆大霉素以及硫酸庆大霉素注射液浓度的测定。结果:成功合成了浓度为2×10-2 mg/L的Mn掺杂ZnS量子点。在pH=7.4的三羟甲基氨基甲烷-盐酸缓冲体系中,静置反应时间为30min条件下,硫酸庆大霉素浓度与Mn掺杂ZnS量子点磷光强度线性关系良好且相关系数R2=0.998 5。结论:该方法灵敏、快捷,可以用于测定尿液中硫酸庆大霉素的浓度。

Abstract:

Objective:Toestablish a simple and sensitive method for the determination of gentamicin sulfate based on the photosensitization of Mn-ZnS quantum dots.Methods:Mercator propionic acid (MPA) modifiedMn-ZnS quantum dots were synthesized in aqueous phase. The effects of pH,type of buffer solution, reaction time and concentration of quantum dots on the room-temperature phosphorescent detection were investigated.The concentrations of gentamicin sulfate and gentamicin sulfate in urine were determined by measuring the phosphorescence intensity of Mn-ZnS quantum dots with a fluorescence spectrophotometer. Results: The Mn-ZnS quantum dots were successfully synthesized. The linear relationship between gentamicin sulfate and phosphorescence intensity of the Mn-ZnS quantum dots (2×10-2 mg/L) was obtained with a correlation of 0.998 5 after 30 min incubation in Tris-HCl buffer (pH=7.4) at room temperature.ConclusionThe proposed method is sensitive and simple and can be used for the determination of gentamicin sulfatein urine.

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金青年基金资助项目(21205087)
作者简介 王娅(1989-)女,药师,学士,研究方向:纳米材料在药物检测方面的应用;通信作者:高卫真,E-mail:weizhengao33@163.com。



 

更新日期/Last Update: 2018-01-23