|本期目录/Table of Contents|

[1]史梦柔,娄建石,李光远,等.正交法制备羧甲基壳聚糖-纳米银纳米微球[J].天津医科大学学报,2018,24(06):496-500.
 SHI Meng-rou,LOU Jian-shi,LI Guang-yuan,et al.Preparation of carboxymethyl chitosan-nano sliver nanoparticles by orthogonal design[J].Journal of Tianjin Medical University,2018,24(06):496-500.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
24卷
期数:
2018年06期
页码:
496-500
栏目:
基础医学
出版日期:
2018-11-20

文章信息/Info

Title:
Preparation of carboxymethyl chitosan-nano sliver nanoparticles by orthogonal design
作者:
史梦柔12娄建石1李光远12郑 夺2陈 斌2高 萍2李毅敏2
(1.天津医科大学药理学教研室,天津300070;2.天津市医药科学研究所医用生物材料监测研究中心,天津300020)
Author(s):
SHI Meng-rou12 LOU Jian-shi1 LI Guang-yuan12ZHENG Duo2CHEN Bin2GAO Ping2 LI Yi-min2
(1.Department of Pharmacology, Tianjin Medical University, Tianjin 300070, China; 2.Medical biological material supervision and research center, Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China)
关键词:
羧甲基壳聚糖纳米银纳米微球离子交联法
Keywords:
carboxymethyl chitosan nano sliver nanoparticles ionic cross-linking method
分类号:
R944.9
DOI:
-
文献标志码:
A
摘要:
目的:制备以纳米银为模型药物的羧甲基壳聚糖载药纳米微球,探索载药纳米微球的制备条件对粒径、包封率的影响,确定最佳制备工艺。方法:三聚磷酸钠为交联剂,采用离子交联法制备载药纳米微球,测量药物的包封率及粒径。紫外分光光度法测定该制剂中纳米银释放情况。结果:通过正交实验设计,优化了制备工艺条件,其最佳条件是羧甲基壳聚糖浓度4.2 mg/mL,滴速为5 s/滴,纳米银投药量为20 mg(500 μL)。在此条件下进行实验,制备出的载药纳米微球的平均粒径为514.1 nm,多分散系数0.204,包封率61.67%。8 h内累计释放量达80%以上,方程拟合以一级动力学方程拟合效果最好。结论:该制备工艺简单、稳定,可制备出包封率高、粒径适宜的羧甲基壳聚糖-纳米银纳米微球。
Abstract:
Objective: To prepare carboxymethyl chitosan drug-loaded nanoparticles with nano silver as model drug the influence of the drug-loaded nanoparticles preparing conditions on the particle size and the encapsulation efficiency to select the optimum conditions for preparation. Methods:With sodium tripolyphosphate as crosslinking agent, Drug-loaded nanoparticles were prepared by ionic cross-Linking method. The drug encapsulation efficiency and particle size were measured. The release of nano-sliver from preparations byultraviolet spectrophotometry was observed. Results:Through the orthogonal design,preparation method was optimized. The optimum conditions included the carboxymethyl chitosan concentration of 4.2 mg/mL,speed of 5 s/drops,and the best dosage of nano sliver for 20 mg(500 μL). In this experiment,the mean size of the preparation drug-loaded nanoparticles was 514.1 nm with the polydispersity index of 0.204,and the encapsulation efficiency was 61.67%.The accumulated amount of release in the preparations is more than 80% at 8 h.Equation of in vitro release model fitted best to Kinetic equation. Conclusion:This preparation method is simple and stable. The carboxymethyl chitosan-nano sliver nanoparticles can be prepared with a high encapsulation efficiency and suitable particle size.

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备注/Memo

备注/Memo:
基金项目 天津市卫生和计划生育委员会科技基金(2013KY38)
作者简介 史梦柔(1988-),女,硕士在读,研究方向:组织工程;通信作者:娄建石,E-mail: Jianshilou@163.com。
更新日期/Last Update: 2018-11-30