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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

24卷

期数:

2018年05期

页码:

404-408

栏目:

基础医学

出版日期:

2018-09-20

文章信息/Info

Title:

Study on the immunomodulatory mechanism of homocysteine in brain tissue injury in spontaneously hypertensive rats

作者:

张 宇; 李 新; 冷 晓; 王 林

天津医科大学第二医院干部保健科, 天津市老年病学研究所, 天津 300211

Author(s):

ZHANG Yu;  LI Xin;  LENG Xiao;  WANG Lin

Department of Geriatrics, The Second Hospital, Tianjin Medical University, Tianjin 300211，China

关键词:

同型半胱氨酸; 自发性高血压大鼠; 免疫机制

Keywords:

homocysteine (Hcy); spontaneously hypertensive rats (SHR);  immune mechanism

分类号:

R544.1

DOI:

-

文献标志码:

A

摘要:

目的：探讨同型半胱氨酸（Hcy）对自发性高血压(SHR)大鼠脑组织损伤的免疫调节机制。方法：取60只SHR大鼠，随机分为SHR-C（对照组）、SHR-M（高蛋氨酸组）和SHR-T（治疗组），每组20只。SHR-C普通饲料喂养，SHR-M给予2 mg/（kg·d）高蛋氨酸饮食。SHR-T组前8周给予2 mg/（kg·d）高蛋氨酸饮食，第9周开始给予叶酸4 mg/（kg·d）、维生素B12（VB12）0.09 mg/（kg·d）、维生素B6（VB6）0.09 mg/（kg·d）灌胃治疗。分别于第0、8、16周检测3组大鼠的体质量、收缩压（SBP）和血浆Hcy浓度。分别于第8、16周用ELISA测血浆中的IL-17和IL-10，实时定量PCR（Q-PCR）检测脑组织中IL-17和IL-10。第16周，免疫组织化学染色法检测脑组织中IL-17和IL-10表达情况。结果：第8周，SHR-M和SHR-T的体质量显著低于SHR-C，血浆Hcy浓度显著高于SHR-T，血浆IL-17浓度显著高于SHR-C，血浆IL-10浓度显著低于SHR-C，脑组织IL-10表达水平显著低于SHR-C（均P<0.05）；SHR-M和SHR-T各参数差异没有统计学意义（P>0.05）。第16周，经治疗后SHR-T组的体质量升高、血浆Hcy浓度下降，与SHR-M组比较具有显著性差异（P＜0.05）；SHR-T组的血浆IL-17浓度降低、IL-10浓度升高，与SHR-M组比较具有显著性差异（P＜0.05）；SHR-T组脑组织IL-17表达水平显著低于SHR-M组，IL-10表达水平显著高于SHR-M组（P>0.05）；SHR-M的脑组织IL-17细胞阳性率明显高于SHR-C和SHR-T，IL-10细胞阳性率明显高于SHR-C和SHR-T。结论：高同型半胱氨酸血症可促进炎症反应从而导致脑组织病变的发生，下调免疫反应后可起到保护作用。

Abstract:

Objective: To explore the immunomodulatory mechanism of homocysteine (Hcy) in brain tissue injury in spontaneously hypertensive rats SHR rats. Methods： Sixty SHR rats were randomly divided into SHR-C (control group), SHR-M (high methionine group) and SHR-T (treatment group), with 20 rats in each group. SHR-C was fed with ordinary diet and SHR-M was given to 2 mg/（kg·d） high methionine diet. SHR-T was given 2 mg/ （kg·d） high methionine diet for the first 8 weeks, and folic acid 4 mg/（kg·d） , vitamin B12 (VB12) 0.09 mg /（kg·d） and vitamin B6 (VB6) were intragastrically administered from week 9. The body weight, systolic blood pressure (SBP) and plasma Hcy concentration in three groups were measured at 0, 8th and 16th weeks, respectively. Enzyme-linked and immunosorbent assay (ELISA) was used to detect the plasma concentration levels of IL-17 and IL-10 at 8th and 16th weeks, and real-time quantitative PCR (Q-PCR) was applied to detected the mRNA levels in the brain tissues. The expression of IL-17 and IL-10 in brain tissue were detected by immunohistochemical staining at week 16. Results： At the 8th week, the body weight of SHR-M and SHR-T was significantly lower than that of SHR-C, and plasma Hcy concentration was significantly higher than that of SHR-T, and plasma IL-17 concentration higher than that of SHR-T, and plasma IL-17 concentration higher than that of SHR-T (all P <0.05). However, there was no significant difference between all parameters of SHR-M and SHR-T at the 8th week (P >0.05). At the 16th week, the body weight of SHR-T increased, and the Hcy concentration decreased after treatment, which were significantly different from those of SHR-M (P < 0.05); the plasma IL-17 concentration of SHR-T decreased and IL-10 concentration increased, which were statistically significant compared with SHR-M (P < 0.05); the mRNA expression level of IL-17 of SHR-T in brain tissue samples was significantly lower than that of SHR-M, and the expression level of IL-10 was significantly higher than that of SHR-M (P >0.05); the positive rate of IL-17 cells of SHR-M was higher than that of SHR-C and SHR-T in the brain tissue (P <0.05), and the positive rate of IL-10 cells was higher. Conclusion： Hcy can promote the inflammatory response and lead to the development of brain tissue, and it can play a protective role after reducing the immune response.

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备注/Memo

备注/Memo:

作者简介 张宇（1982-），男，主治医师，博士，研究方向：老年医学；通信作者：王林，E-mail: wang lin@medmail.com.cn。

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更新日期/Last Update: 2018-09-30