|本期目录/Table of Contents|

[1]徐灵灵,牛秀珑,张宏健,等.IL-17A促进卵巢癌顺铂耐药的体外机制探讨[J].天津医科大学学报,2018,24(03):192-196.
 XU Ling-ling,NIU Xiu-long,ZHANG Hong-jian,et al.Study on the underlying mechanisms of IL-17A promoting the cisplatin-based resistance of ovarian cancer[J].Journal of Tianjin Medical University,2018,24(03):192-196.
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IL-17A促进卵巢癌顺铂耐药的体外机制探讨(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
24卷
期数:
2018年03期
页码:
192-196
栏目:
出版日期:
2018-05-20

文章信息/Info

Title:
Study on the underlying mechanisms of IL-17A promoting the cisplatin-based resistance of ovarian cancer
作者:
徐灵灵1牛秀珑2张宏健3李稚君4陈燕1邓为民1
1.天津医科大学免疫学系,天津300070;2.中国人民武装警察部队后勤学院附属医院感染性疾病科, 天津300162;3.天津医科大学总医院输血科,天津300052;4.天津医科大学总医院骨科,天津300052
Author(s):
XU Ling-ling1 NIU Xiu-long2 ZHANG Hong-jian3 LI Zhi-jun4 CHEN Yan1 DENG Wei-min1
1. Department of Immunology, Tianjin Medical University, Tianjin 300070, China; 2. Department of Infectious Diseases, Hospital Affiliated to Logistics College of Chinese People’s Armed Police Forces, Tianjin 300162, China; 3. Department of Blood Transfusion, General Hospital, Tianjin Medical University, Tianjin 300052, China; 4. Department of Orthopedics, General Hospital, Tianjin Medical University, Tianjin 300052, China
关键词:
卵巢癌IL-17A顺铂耐药
Keywords:
ovarian cancer IL-17A cisplatin drug-resistance
分类号:
R392.1
DOI:
-
文献标志码:
A
摘要:
目的:探究IL-17A促进卵巢癌顺铂(DDP)耐药的体外机制。方法:应用流式细胞术分析外源性rhIL-17A对DDP诱导的A2780(顺铂敏感卵巢癌细胞株)和OVCAR3(顺铂耐药卵巢癌细胞株)细胞的凋亡和周期分布变化的影响,并以IL-17RAmAb进行相应的阻断实验。结果:rhIL-17A对A2780和OVCAR3细胞的凋亡无显著影响;但可显著降低DDP对A2780和OVCAR3细胞的毒性作用(P<0.05),以IL-17RAmAb进行阻断实验可部分消除rhIL-17A对DDP诱导的A2780和OVCAR3细胞凋亡的阻滞作用(P<0.05)。rhIL-17A对A2780和OVCAR3细胞的周期分布无显著影响;但可显著抑制 DDP诱导的A2780和OVCAR3细胞周期阻滞(P <0.05),使A2780和OVCAR3细胞的G0/G1期比例增加,G2+S期比例减少。结论: IL-17A可通过IL-17RA抑制DDP诱导的卵巢癌细胞凋亡,同时可抑制DDP诱导的卵巢癌细胞周期阻滞,从而加剧以DDP为基础的卵巢癌化疗耐药性。
Abstract:
Objective: To explore the underlying mechanisms of IL-17A promoting the cisplatin-based resistance of ovarian cancer. Methods: Flow cytometry assay was used to detect the effect of exogenous rhIL-17A on the cisplatin-based cell apoptosis and cycle distribution of A2780 and OVCAR3 cells, and then neutralizing IL-17RAmAb was applied to perform the blocking test. Results: RhIL-17A alone had no effect on the apoptosis of A2780 and OVCAR3 cells, but it could significantly inhibit the cytotoxicity of DDP to A2780 and OVCAR3 cells (P <0.05). Furthermore, neutralizing IL-17RAmAb could markedly block the above effect of rhIL-17A on DDP-sensitivity (P <0.05). There was no effect of rhIL-17A on cell cycle distribution of A2780 and OVCAR3 cells. After pre- treatment with rhIL-17A, the DDP effect on cell cycle might be changed partially by increasing the G0/G1 phase and decreasing the G2+S phase (P <0.05). Conclusion: IL-17A could inhibit the cisplatin-based cell apoptosis of ovarian cancer cell, partially through IL-17RA signal pathway. Moreover, IL-17A could also repress the effects of DDP on cell cycle distribution of ovarian cancer cell, which may provide a novel strategy to improve the chemoresistance of ovarian cancer.

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备注/Memo

备注/Memo:
文章编号 1006-8147(2018)03-0192-05
基金项目 天津市教委重点项目(2016YD01);天津市科委应用基础研究项目(15JCYBJC26000);武警后勤学院博士启动金项目资助(WHB201610)
作者简介 徐灵灵(1990-),女,硕士在读,研究方向:肿瘤免疫;通信作者:邓为民,E-mail:dengweimin@tmu.edu.cn。
更新日期/Last Update: 2018-05-20