|本期目录/Table of Contents|

[1]廖健文,岳莹莹,牛文彦.AMPK对Rab-GAP的调节作用研究[J].天津医科大学学报,2018,24(02):97-100.
　LIAO Jian-wen,YUE Ying-ying,NIU Wen-yan.Study on the regulation of Rab-GAP by AMPK[J].Journal of Tianjin Medical University,2018,24(02):97-100.
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AMPK对Rab-GAP的调节作用研究(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
24卷
期数:
2018年02期
页码:
97-100
栏目:
出版日期:
2018-03-20

文章信息/Info

Title:
Study on the regulation of Rab-GAP by AMPK
作者:
廖健文; 岳莹莹; 牛文彦
天津医科大学免疫学系，天津300070
Author(s):
LIAO Jian-wen;  YUE Ying-ying;  NIU Wen-yan
Department of Immunology, Tianjin Medical University, Tianjin 300070, China
关键词:
单磷酸腺苷激活蛋白激酶; TBC1D1; TBC1D4; 磷酸化
Keywords:
AMPK;  TBC1D1;  TBC1D4;  phosphorylation
分类号:
R392.1
DOI:
-
文献标志码:
A
摘要:
目的：检测激活型AMPK对Rab-GAP的调节作用，明确它们之间的上下游关系。方法：激活型AMPK腺病毒（Ad-AMPK-CA）感染C2C12小鼠骨骼肌细胞，通过细胞成像系统测定细胞中的荧光强度，明确AMPK-CA在C2C12细胞中表达。MTS试验确定没有细胞毒性的腺病毒浓度，Western blot检测AMPK底物ACC和两个Rab-GAP（TCB1D1和TCB1D4）的磷酸化。结果：AMPK-CA 显著升高ACC的磷酸化，并磷酸化TBC1D1 Thr590和TBC1D4 Ser318。结论：AMPK直接磷酸化TCB1D1和TCB1D4。
Abstract:
Objective: To examine the effect of constitutive active AMPK on Rab-GAP phosphorylaiton to elucidate the signal relationship. Methods: Mouse C2C12 skeletal muscle cells were infected with constitutive active AMPK adenovirus (Ad-AMPK-CA). The expression of AMPK in C2C12 cells was observed by cell fluorescence imaging system. Cytotoxicity of the adenovirus was determined by MTS. Phosphorylations of AMPK substrate ACC and two Rab-GAPs (TCB1D1 and TCB1D4) were detected by Western blot. Results: AMPK-CA significantly elevated the phosphorylations of ACC and TBC1D1 Thr590 and TBC1D4 Ser318. Conclusion: AMPK directly could regulate TBC1D1 and TBC1D4.

参考文献/References:

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备注/Memo

备注/Memo:
文章编号 １００６－８１４７（２０18）０2-0097-04
基金项目 国家自然科学基金资助项目（81170740）；高等学校博士学科点专项基金资助项目（20121202110014）；天津市科委应用基础研究重点项目（15JCZDJC35500）；天津市卫计委重点攻关项目（15KG102）
作者简介 廖健文（1989-），女，硕士在读，研究方向：免疫学；通信作者：牛文彦，E-mail:wniu@tmu.edu.cn。
更新日期/Last Update: 2018-03-20