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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

20卷

期数:

2014年04期

页码:

267-270

栏目:

基础医学

出版日期:

2014-07-08

文章信息/Info

Title:

Effects of hydrogen–rich perfusion on lung injury by hepatic cold ischemia／reperfusion in rat

文章编号:

1006-8147（2014）04-0267-04

作者:

巫 强¹ ; 朱志军²; 徐 倩¹; 丁 梅¹; 王玉亮³

(1．天津医科大学研究生院，天津300070；2．北京友谊医院肝移植病房，北京 100050；3．天津市卫生部危重病急救医学重点实验室，天津300384)

Author(s):

WU Qiang¹;  ZHU Zhi-jun²;  XU Qian¹;  DING Mei¹;  WANG Yu-liang³

(1．Graduate School, Tianjin Medical University, Tianjin 300070, China；2．Departmennt of Liver Transplantation, Beijing Friendship Hospital , Beijing 100050, China；3．The Key Laboratory of Critical Care of Medicine Ministry of Health, Tianjin 300384, China)

关键词:

氢; 肝; 肺; 氧化应激; 缺血再灌注损伤; 大鼠

Keywords:

LINE-HEIGHT: 18pt;  TEXT-ALIGN: left" align="left">hydrogen; liver; Pulmonary ; oxidative stress; ischemia/reperfusion injury; rat

分类号:

R617

DOI:

-

文献标志码:

A

摘要:

目的：评价含饱和氢气乳酸林格灌注液对大鼠肝脏冷缺血再灌注所引起的肺损伤的影响。方法：健康雄性Wistar大鼠24只，随机分为对照组（S组）、普通灌注液组（I/R组）及含饱和氢气灌注液组（H组），每组8只分为3组给予相应实验处理。3组大鼠均于再灌注6 h后经下腔静脉采血离心获取血清，测定血清中肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）的浓度。经腹主动脉采血监测动脉血气。取血后处死大鼠获取肺脏，检测肺组织丙二醛（MDA）的含量及超氧化物歧化酶（SOD）的活性，光镜下观察肺组织病理学改变。结果：血清中TNF-α、IL-6、IL-1β水平为I/R组>H组>S组（P<0.05）；肺组织中MDA浓度为I/R组>H组>S组（P<0.05）；而肺组织中SOD活性S组>H组>I/R组（P<0.05）。各组动脉血PaO2水平为S组>H组>I/R组（P<0.05），各组中PaCO2 差异无统计学意义（P>0.05）。S组肺组织形态结构未见明显异常，I/R、H组均见肺组织损伤，H组损伤较I/R组减轻。结论：含饱和氢气乳酸林格灌注液可以降低炎性反应抑制机体脂质过氧化反应，减轻大鼠肝脏冷缺血再灌注肺损伤，改善肺功能。

Abstract:

Objective: To evaluate the effects of hydrogen-rich Lactate Ringer’s perfusion solution on lung injury caused by hepatic cold ischemia/reperfusion in rats. Methods: Twenty-four healthy male Wistar rats, were randomly divided into control group (S group), common perfusion solution group (I/R group), hydrogen-rich liver perfusion solution group (H group) with 8 in each, before corresponding experimental treatment was given. Six hours after reperfusion, serum was obtained by centrifuging the venous blood to detect the level of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β). Abdominal aortic blood was taken to monitor arterial blood gas. Lungs of all rats in three groups were removed after venous blood collection from inferior vena cava to detect the level of malondialdehyde (MDA) and superoxide dismutase (SOD) in lungs. The pathological changes of lung were evaluated by light microscopy. Results: The levels of TNF-α, IL-6 and IL-1β in serum ranked I/R group > H group > S group (P<0.05); And the descending order of MDA concentrations in the lung tissue was I/R group > H group > S group (P<0.05); And for SOD activity of lung tissue ， S group >Group H> I/R group (P<0.05). The levels of arterial blood PaO₂ ranked group S > H group > I/R group (P < 0.05) with no statistically significant difference between groups of PaCO₂ (P > 0.05). The modality and structure of lung tissue in group S were normal. The injury in groups I/R and H were obvious, and the injury in group H was alleviated compared to group I/R. Conclusion: Hydrogen-rich Lactate Ringer’s liver perfusion solution can reduce the lung injury caused by hepatic cold ischemia/reperfusion injury.

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相似文献/References:

备注/Memo

备注/Memo:

基金项目 天津市卫生行业重点攻关项目 (12KG101), 国家高技术研究发展计划（2012AA021001）

作者简介 巫强（1987-），男，硕士在读；研究方向：肝移植；通信作者：朱志军，E-mail：zhu-zhijun@medmail.com.cn。

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更新日期/Last Update: 2014-06-06